Mobilization of Genomic Islands of Staphylococcus aureus by Temperate Bacteriophage

Moon, B.Y., Park, J. Y., Robinson, D. Ashley, Thomas, J.C., Park, Y. H., Thornton, J.A. and Seo, K. S. (2016) Mobilization of Genomic Islands of Staphylococcus aureus by Temperate Bacteriophage. PLoS ONE, 11 (3).

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Abstract

The virulence of Staphylococcus aureus, in both human and animal hosts, is largely influenced by the acquisition of mobile genetic elements (MGEs). Most S. aureus strains carry a variety of MGEs, including three genomic islands (νSaα, νSaβ, νSaγ) that are diverse in virulence gene content but conserved within strain lineages. Although the mobilization of pathogenicity islands, phages and plasmids has been well studied, the mobilization of genomic islands is poorly understood. We previously demonstrated the mobilization of νSaβ by the adjacent temperate bacteriophage ϕSaBov from strain RF122. In this study, we demonstrate that ϕSaBov mediates the mobilization of νSaα and νSaγ, which are located remotely from ϕSaBov, mostly to recipient strains belonging to ST151. Phage DNA sequence analysis revealed that chromosomal DNA excision events from RF122 were highly specific to MGEs, suggesting sequence-specific DNA excision and packaging events rather than generalized transduction by a temperate phage. Disruption of the int gene in ϕSaBov did not affect phage DNA excision, packaging, and integration events. However, disruption of the terL gene completely abolished phage DNA packing events, suggesting that the primary function of temperate phage in the transfer of genomic islands is to allow for phage DNA packaging by TerL and that transducing phage particles are the actual vehicle for transfer. These results extend our understanding of the important role of bacteriophage in the horizontal transfer and evolution of genomic islands in S. aureus.

Item Type: Article
Additional Information: This work was partially supported by grants from National Institute of Food and Agriculture, United States Department of Agriculture (2008-35204- 04582) to K.S.S.; Center for Biomedical Research Excellence in Pathogen-Host interactions, National Institute of General Medical Sciences, NIH (1P20GM103646-01A1) to K.S.S.; Animal and Plant Quarantine Agency, South Korea Department of Agriculture to K.S.S; National Institute of General Medical Sciences, NIH (GM080602) to D.A.R. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Divisions: ?? biology ??
Depositing User: Tracey Gill
Date Deposited: 03 Oct 2016 17:45
Last Modified: 07 Feb 2017 10:37
Identification Number: 10.1371/journal.pone.0151409
URI: http://ubir.bolton.ac.uk/id/eprint/940

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