Graphene oxide for electrochemical sensing applications

Roy, Soumyendu, Soin, Navneet ORCID: 0000-0002-0196-2071, Bajpai, Reeti, Misra, DS, McLaughlin, James A and Roy, Susanta Sinha (2011) Graphene oxide for electrochemical sensing applications. Journal of Materials Chemistry, 21 (38). pp. 14725-14731. ISSN 0959-9428

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By exploiting the presence of abundant carboxylic groups (–COOH) on graphene oxide (GO) and using EDC–NHS (1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride–N-hydroxysuccinimide) chemistry to covalently conjugate protein molecules, we demonstrate a novel electrochemical immunosensor for detection of antibody–antigen (Rabbit IgG–AntiRabbit IgG) interactions. The interactions were verified using Electrochemical Impedance Spectroscopy (EIS). Although GO is known to be a poor conductor, the charge transfer resistance (RP) of a GO modified glassy carbon electrode (GCE) was found to be as low as 1.26 Ω cm2. This value is similar to that obtained for reduced graphene oxide (RGO) or graphene and an order of magnitude less than bare GCE. The EIS monitored antibody–antigen interactions showed a linear increase in RP and the overall impedance of the system with increase of antibody concentration. Rabbit IgG antibodies were detected over a wide range of concentrations from 3.3 nM to 683 nM with the limit of detection (LOD) estimated to be 0.67 nM. The sensor showed high selectivity towards Rabbit IgG antibody as compared to non-complementary myoglobin. RGO modified GCE showed no sensing properties due to the removal of carboxylic groups which prevented subsequent chemical functionalization and immobilization of antigen molecules. The sensitivity and selectivity achievable by this simple label free technique hint at the possibility of GO becoming the electrode material of choice for future electrochemical sensing protocols.

Item Type: Article
Subjects: Q Science > QD Chemistry
Divisions: University of Bolton Research Centres > Institute for Materials Research and Innovation
Depositing User: Dr Navneet Soin
Date Deposited: 28 Mar 2018 14:34
Last Modified: 28 Mar 2018 14:34
Identification Number: 10.1039/C1JM12028J

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